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DEPARTMENT OF VET. GYNAECOLOGY & OBSTETRICS
Faculty Members
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Name
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Designation
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Qualifi-
cations
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Specialization
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Contact No.
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Email
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Dr. H.P. Gupta
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Prof. & Head
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Ph.D.
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Animal
Reproduction
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05944-233067
(O)
9411329387
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hpguptavgo[at]gmail[dot]com
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Dr.
A.K. Misra*
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Professor
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Ph.D.
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Animal
Reproduction
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9410606134
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prof[underscore]misra[at]hotmail[dot]com
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Dr.
Shiv Prasad
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Professor
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Ph.D.
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Animal
Reproduction
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9411377368
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shivp2003[at]yahoo[dot]co[dot]uk
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Dr.
Mridula Sharma
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Asstt. Professor
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Ph.D.
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Animal
Reproduction
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9412462579
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sharmavetmridula[at]yahoo[dot]co[dot]in
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* On extra ordinary leave/joined as Vice-Chancellor, Maharashtra Animal &
Fishery Sciences University, Nagpur.
Courses offered by the Department
B.V.Sc.
& A.H. Courses
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S. No.
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Name of the Course
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Course No.
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Credit Hrs.
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1.
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Veterinary
Gynaecology
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VGO 411
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3 (2 +1)
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2.
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Veterinary
Obstetrics
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VGO 421
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2 (1+1)
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3.
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Veterinary
Andrology & Reproductive Techniques
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VGO- 511
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2 (1+1)
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M.V.Sc.
Courses
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S. No.
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Name of the Course
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Course No.
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Credit Hrs.
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1.
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Seminar
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VGO 600
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1
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2.
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Special
Problem
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VGO 601
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1 (0+1)
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3.
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General
Gynaecology
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VGO 611
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4 (3+1)
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4.
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Female Infertility
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VGO 612
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4 (3+1)
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5.
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Veterinary Obstetrics
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VGO 613
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4 (3+1)
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6.
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Andrology & Male
Infertility
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VGO 614
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4 (3+1)
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7.
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Semen Preservation
And Artificial Insemination
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VGO 615
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3 (2+1)
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8.
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Reproductive
Biotechnology
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VGO 616
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3 (2+1)
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9.
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Clinical
Practice 1
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VGO 617
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1 (0+1)
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10.
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Clinical
Practice 1I
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VGO 618
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1 (0+1)
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11.
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Master’s Research
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VGO 690
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20
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Ph.D.
Courses
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S. No.
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Name of the Course
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Course No.
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Credit Hrs.
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1
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Advances in Gynaecology
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VGO 701
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3 (2+1)
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2.
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Advances in Obstetrics
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VGO 702
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3 (2+1)
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3.
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Advances in Andrology
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VGO 703
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3 (2+1)
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4.
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Advances in Reproductive Biotechnology
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VGO 704
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2 (1+1)
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5.
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Advances in Semen Preservation
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VGO 705
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2 (1+1)
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6.
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Advanced Clinical
practice I
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VGO 706
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1 (0+1)
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7.
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Advanced Clinical
practice II
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VGO 707
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1 (0+1)
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8.
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Doctoral
Seminar 1
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VGO 788
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1 (1+0)
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9.
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Doctoral
Seminar 1I
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VGO 789
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1 (1+0)
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10.
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Ph.D.
Thesis Research
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VGO 790
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45
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Salient Achievements:
1. Embryo Biotechnology
i. The Department is pioneer in initiating embryo transfer work in
rabbit and goat in the year 1976.
ii. Initial trial of surgical transfer of goat embryos revealed 63.50% embryo
survival. Goat embryos were also frozen using 2.00 MDMSO.
iii. Super-ovulation studies were performed in buffalo using PMSG.
iv. Super-ovulation, non-surgical embryo collection and non-surgical embryo
transfers were performed for standardization of various aspects of embryo
transfer technology including pregnancy rate in Sahiwal and Crossbred cows.
Several Sahiwal and Crossbred calves, including seven calves from one cow,
were produced.
v. Research trials were conducted to study effect of various gonadotropins
and endocrine profile during superovulatory estrus to improve superovulatory
response and embryo recovery. The mean total and viable embryo recovery of
upto 5.25 and 1.80 per flushing, respectively was achieved. In Sahiwal cows,
the mean ovulation rate, total and viable embryo recovery was observed to the
9.76, 3.52 and 1.67, respectively.
vi. The mean pregnancy rate on transfer of fresh and frozen embryos was 23.80
and 36.40%, respectively. The improved conception was observed with GnRH
treatment to recipients on day 5 and also with transfer of fresh embryos of
excellent grade (upto 76.90% conception).
vii. Extensive studies ware made to study in-vitro maturation (IVM) and
in-vitro fertilization (IVF) of oocytes, in vitro capacitation of sperm, and
in-vitro culture (IVC) of embryos using various media and co-culture systems to
study the media requirement for oocytes/embryos of different stages. Immature
oocytes were also vitrified successfully using different cryo-protective
agents.
viii. The technique of biweekly collection of ultrasound guided trans-vaginal
oocyte aspiration (ovum pick-up) in Sahiwal cows has been established and
embryos are being produced using IVM/IVF and IVC technology.
2. Semen Biotechnology
Extensive studies were undertaken in the area of Andrology, Artificial
Insemination and Semenology
including qualitative and quantitative aspects.
i. Studies on Semenology aspects of Crossbred bulls of different
breed/genetic Friesian are not good producers, especially when the exotic
inheritance increased more than constitution revealed that Crossbred bulls
having exotic inheritance of Red Dane and Holstein-62.50%. Crossbred bulls
with 50.00% exotic inheritance were better. There was non-significant effect
of seasons on semen production potential of these bulls.
ii. Different modifications of egg yolk tris dilutor (EYT) were successfully
developed to cryopreserve cattle and
buffalo semen. Foetal calf serum (10.00%), caffeine (1.00mM), insulin
(10.00µg/ml) and ascorbic acid (45.00µM) singly and/or in various combinations
were found suitable additives to EYT for cryopreservation of bovine and
bubaline semen.
iii. The antioxidant n-propyl gallate (15.00µM), stimulants-caffeine
(1.00mM) and Bradykinin (2.00ng/ ml) and membrane stabilizer Demecolcin
(0.50µg/ml) had significant beneficial effect on buffalo semen
cryopreservation and had better in-vitro fertility. Use of buffalo semen
frozen in modified dilutor (Tris + 15.00µM n-propyl gallate + 1.00mM caffeine
+ 0.50 µg/ ml Demecolcin) under field conditions, revealed 52.00% conception
(n=75) compared to 45.00% conception (n=119) using control (Tris) dilutor.
iv. During investigation into bacterial load of semen and suitable
antibiotic, it was concluded that streptomycin (800.00µg per ml) and
penicillin (800.00 IU per ml) was the minimum effective dose to reduce the
bacterial load to permissible level in frozen semen of cattle and buffalo.
Minimum and maximum dose of amikacin for bovine frozen semen was 100.00 and
2500.00 µg/ml. Corresponding values for buffalo were 250.00 and 2000.00
µg/ml. December witnessed minimum bacterial load in cattle and buffalo semen
ejaculate, while maximum one was in August.
v. FMD vaccination of cattle and buffalo bulls, adversely affected the semen
quality, which returned to normal in 60-75 days.
3. Fertility-infertility
i. While studying different aspects of female fertility, it was found
that proteins and sialic acid in estrual mucus of Crossbred and Sahiwal cows
and Murrah buffaloes were higher in normal animals than in repeat breeders
and they were responsible for higher conception rate. However, progesterone
level differed non-significantly between normal and repeat breeding animals.
ii. Incidence of retained placenta was the highest in cattle during
winter followed by metritis and dystokia. However, metritis was witnessed
maximum in buffaloes followed by RP, dystokia and vaginal prolapse. Overall
incidences of reproductive disorders were maximum (45-89.00%) in rainy
season. Studies on caesarotomy in dystokia cases revealed that most of
operated cows and buffaloes attained normal health status in 168 hrs.
iii. Emetic nuts were found significantly (P<0.01) effective remedy to
treat anestrus, as 88.80% of the treated cows exhibited estrus as compared to
only 15.00% in control.
iv. Different drugs were tested to select suitable therapeutic package
to treat anestrum in buffaloes. The overall recovery rate with Receptal
treatment was 85.71% at Livestock
Research Center
of the University and 50.00% in field buffaloes compared to 0.00 and 12.50%
recovery in control groups, respectively. The overall recovery with Crestar
treatment on anoestrous buffaloes was 62.50% in L.R.C. buffaloes and 87.50%
in field buffaloes compared to 2.50% recovery in control groups both in
L.R.C. and field buffaloes. The anoestrous recovery in field buffaloes ranged
from 75.00% to 87.50% with CIDR treatment. The CIDR treatment was found to be
the most suitable.
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